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HPTLC-Densitometric Method for Estimation of in Bulk, Pharmaceutical Formulations

Journal of Scientific and Technical Research

Volume 6 Issue 2

Published: 2016
Author(s) Name: Aradhana Sharma, Kumar Gaurav, Ram Singh, Richa Srivastava | Author(s) Affiliation:
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Abstract

A simple, sensitive and precise HPTLC (high performance thin layer chromatography) method has been developed for quantitative estimation of ketamine in bulk and pharmaceutical formulations. The separation of ketamine was achieved over HPTLC glass plate of silica GF- 254 by using cyclohexane:toluene: diethylamine (5:3:2 v/v) solvent system. Densitometric evaluation was carried out in the UV region at wavelength of 254. The method was found to be accurate (98.92–100.26%) and precise (CV = 0.43–0.67 for intra-day and CV = 0.58–1.45 for inter-day) within the corresponding linear range. The limit of detection (LOD) and limit of quantification (LOQ) of sample have been found to be 100 ng/spot and 303 ng/spot, respectively.

Keywords: Date Rape Drug, Densitometric Method, Drug of Abuse, General Anesthetic, Hallucinogen, HPTLC, Ketamine

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