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In-Vitro Binding Interaction of 3-(Furan-2-Yl)-N-Phenylprop-2-Enamide With BSA: Equilibrium Dialysis and FT-IR Spectroscopic Study

Journal of Scientific and Technical Research

Volume 9 Issue 2

Published: 2019
Author(s) Name: Ajay M. Pisudde, Pradip V. Tekade and Shrikant B. Thakare | Author(s) Affiliation: Department of Chemistry, Jankidevi Bajaj College of Science, Wardha, Maharashtra, India
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Abstract

This paper presented binding interaction of 3-(Furan-2-yl)-N-phenylprop-2-enamide (3FNPP2M) with bovine serum albumin (BSA) by equilibrium dialysis and FT-IR spectroscopy at physiological pH in various solvents. Findings were interpreted by scatchard plot in terms of association constants. The increased in association constants with increase in concentrations of the 3FNPP2M is observed. The binding interaction is found to be more in 1, 4-dioxane than DMSO and DMF.FT-IR study explained the binding interaction through shift in peak positions of amide I and II bands. FT-IR study confirmed the binding of 3FNPP2M with BSA in terms of changes in secondary structure of BSA. In this study, we have also reported the effect of foreign particles viz. arsenic and mercury on the binding interaction of 3FNPP2M with BSA. Reactivity and association of 3FNPP2M with BSA in presence of foreign moieties were calculated and compared.

Keywords: Association constant, BSA, Equilibrium dialysis, Foreign particles, FT-IR study, Scatchard plot.

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