Variation in Antioxidant Activity and Antioxidant Constituents in Different Extracts of Bacopa monnieri Linn. with the Plant Maturity, Grown in Middle Hill Climatic Condition Western Himalayas
Published: 2023
Author(s) Name: Hemant Kumar Pandey, Anchala Guglani, Swati, Vinod Kumar and Arun Kumar Maurya |
Author(s) Affiliation: Defence Institute of Bio-Energy Research (DRDO), Pithoragarh, Uttarakhand, India
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Abstract
A comparison of the antioxidant components and antioxidant activity of Bacopa monnieri Linn. in aqueous and alcoholic extracts at different growth stages (in every 15 days interval) till plant maturity (till 90 days) was carried out. In addition, a correlation analysis between antioxidant activity and antioxidant components was also performed. The research exhibited that the alcoholic extract contains greater antioxidant constituents with maximum antioxidant activity in comparison to the aqueous extracts. The results showed that the 15 days old plant exhibited the highest antioxidant activity with minimum IC50/EC50 value by different antioxidant assays, such as DPPH (0.087 mg/ml), ABTS (0.084 mg/ml), and FRAP (0.614 mg/ml) in the alcoholic extract. In correlation analysis also found that the ABTS assay’s IC50 value had a considerably positive correlation with the DPPH and FRAP tests’ IC50 and EC50 values, respectively, and that antioxidant components had a negative correlation with IC50/EC50. In conclusion, the study found that 15-day-old plant extracts have the best antioxidant potential and the highest concentration antioxidant components when compared to other plant growth stages.
Keywords: ABTS, Antioxidant constituents, Correlation, Growth stages, IC50 value.
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