The ASHA workers are for primary healthcare services, especially in rural areas. Despite being not paid properly, neglected, and not recognized, their personality and the organization’s roles motivated ASHAs to perform their roles and responsibilities. The study aims to analyze the mediation of personality in the relationship between organizational roles and motivation. The study is both qualitative and quantitative, the data being collected from 140 ASHAs of 10 PHC centers in Bangalore, identified based on a stratified sample and through a questionnaire, face-to-face interviews, and focus group discussions in November and December 2022. The study reveals that there is a complete mediation effect of personality on the relationship between organizational roles and Motivation. The findings of the study are useful for NRHM and PHC centers to redesign their policies and strategies.